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method of screening plant extract with diuretic potential

method of screening plant extract with diuretic potential

Evaluation of antioxidant and diuretic activities of ...

2021-1-6  In the present study, the diuretic and antioxidant potential of Clitoria ternatea leaf extracts were evaluated. Preliminary phytochemical screening of three solvent extracts [methanol (MCTL), chloroform, and aqueous) of the leaves revealed that the extracts were rich in alkaloids, glycosides, carbohydrates, flavonoids, and saponins. Among the ...

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Biological screening of various medicinal plant extracts ...

screening and testing plant extracts (4). Extracts from a broad spectrum of plant species contain substances that possess antitumor activity (1). New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities (5). Increasingly, natural products have potential for the treatment of cancer.

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Phytochemical screening and in vitro bioactivity of ...

2015-9-19  Phytochemical screening for major constituents was undertaken us-ing standard qualitative methods as described by Sofowora (1993) method. The test for tannins was carried out by subjecting 3 g of each plant extract in 6 ml of distilled water, filtered and ferric chlo-ride reagents added to the filtrate. For cardiac glycosides, Killer-

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Evaluation of diffusion and dilution methods to

We suggest evaluation of the antibacterial activity of plant extracts using the broth microdilution method as a fast screening method for MIC determination and the macrodilution method at selected MIC values to confirm bacterial inactivation.

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PRELIMINARY PHYTOCHEMICAL SCREENING OF SIX

2020-12-29  Watal et al. Int J Pharm Pharm Sci, Vol 6, Issue 5, 539-542 540 Saponins: Persistent frothing on warming the extract of S. chirata indicated the presence of saponins in this plant only. The same extract with few drops of olive oil formed a soluble emulsion,

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Phytochemical screening and in vitro evaluation of ...

2017-10-1  The scavenging ability of the plant extract was then calculated using the following equation: DPPH Scavenging activity (%) = [(Abs control – Abs sample)/(Abs control)] × 100 In this equation, Abs control is the absorbance of DPPH + methanol; Abs sample is the absorbance of DPPH radical + sample/or standard. 2.5.3.

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Evaluation of diuretic and laxative activity of hydro ...

2014-7-1  The hydro-alcoholic extract of D. bipinnata whole plant was prepared by using Soxhlet exPulverizer and subjected to analysis by standard preliminary phytochemical tests. Evaluation of both diuretic and laxative activity was carried out using standard methods as reported earlier. Frusemide (20 mg/kg) was served as positive control for diuretic activity ...

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Comparative study of diuretic potential of aqueous and ...

The diuretic activity of extracts of Fumaria indica was examined by treating different groups of wistar albino rats with single (200 mg/kg) oral doses of alcoholic and aqueous extract/fractions ...

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Antimicrobial potentials of medicinal plant’s extract

2019-11-1  Recently, seven medicinal plants were examined for antibacterial potential, the result indicated that the methnolic extract of Phyllanthus niruri (stone breaker) was to be found strong action against Staphylococcus sp, while the aqueous and methanolic fraction had minimum activity as compared to methanolic (Selvamohan et al., 2012). The author used total six plants, against dental pathogens.

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Effect of Extraction Methodologies on Antioxidant ...

2018-3-3  The solvents used to play a significant role on biological activities of plant extracts regardless of the method of extraction used. The crude ethanolic extract has higher anticancer potential against colon cancer cell line HCT-116 as compared to other extracts and standard gallic acid that is reported in this plant species.

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Evaluation of antioxidant and diuretic activities of ...

2021-1-6  In the present study, the diuretic and antioxidant potential of Clitoria ternatea leaf extracts were evaluated. Preliminary phytochemical screening of three solvent extracts [methanol (MCTL), chloroform, and aqueous) of the leaves revealed that the extracts were rich in alkaloids, glycosides, carbohydrates, flavonoids, and saponins. Among the ...

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Phytochemical screening and in vitro bioactivity of ...

2015-9-19  extract of the plant. The presence of phlobatannins sug-gests the diuretic property of the plant (Okuda, 1991). The foregoing would suggest the possible utilization of C. aconitifolius as diuretic agent. It was not really surprising that flavonoids were found absent for both forms of extracts. The sole greenish appearance of this leafy

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Biological screening of various medicinal plant extracts ...

screening and testing plant extracts (4). Extracts from a broad spectrum of plant species contain substances that possess antitumor activity (1). New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities (5). Increasingly, natural products have potential for the treatment of cancer.

Get Price

Screening for Potential Bioactive Components in

The overall findings indicate that 8 prototype compounds may be the potential bioactive components of GBE with preventive effect against DN and the method of RTEC extraction coupled with LC-MS/MS technology screening method we developed is a feasible, rapid, and useful tool for screening and analyzing potential bioactive components.

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Phytochemical screening and in vitro evaluation of ...

2017-10-1  H 2 O 2 scavenging activity of the plant extract was determined by the method of Owaisi et al. [17] with slight modification. Briefly, plant extracts (1 mL) at different concentrations were mixed with 0.6 mL of 4 mM H 2 O 2 solution prepared in phosphate buffer (0.1 M pH 7.4) and incubated for 10 min.

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Evaluation of diuretic and laxative activity of aqueous ...

2021-6-15  to evaluate the extract for its diuretic and laxative potential. Method: The aqueous extract of Argemone mexicana prepared using percolation method and subjected to phytochemical analysis. Evaluation of diuretic and laxative activity was carried out using metabolic cage apparatus and flame photometer as per the standard method reported earlier.

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Evaluation of diffusion and dilution methods to

The disk diffusion method was appropriate only as a preliminary screening test prior to quantitative MIC determination with dilution methods. A comparison of the results for MIC obtained by agar dilution and broth microdilution was possible only for gram-positive bacteria, and indicated the latter as the most accurate way of assessing the ...

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Qualitative analysis of phytochemicals and anti

2020-1-1  The antibacterial potential of the extracts were evaluated on gram-positive (Bacillus subtilis) and gram-negative (Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas pyogens) bacteria in terms of zone of inhibition using agar well diffusion method.In the anti-bacterial screening, it is evident that flower has more anti-bacterial property ...

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Antimicrobial potentials of medicinal plant’s extract

2019-11-1  The extract of Sphareranthu hirtus was the most active against multi-drug resistant Pseudomonas aeruginosa and enterohemorrhagic E. coli. The ethanolic extract of S. hirtus exhibited a higher effect than the hot water extract (Hassan et al., 2009). The author investigated six plants leaves against Klebsiella, Pseudomonas and E. coli.

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INDIAN MEDICINAL PLANTS WITH DIURETIC ACTIVITY

2020-5-8  Plant part used: Whole plant The methanolic extract of whole plant of Achyranthes aspera was investigated for its diuretic potential. The diuretic effect was found out by Lipschitz et al method using furosemide as standard drug. [8] Diuretic activity of aqueous extract

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Screening for Potential Bioactive Components in

The overall findings indicate that 8 prototype compounds may be the potential bioactive components of GBE with preventive effect against DN and the method of RTEC extraction coupled with LC-MS/MS technology screening method we developed is a feasible, rapid, and useful tool for screening and analyzing potential bioactive components.

Get Price

Phytochemical screening and in vitro evaluation of ...

2017-10-1  H 2 O 2 scavenging activity of the plant extract was determined by the method of Owaisi et al. [17] with slight modification. Briefly, plant extracts (1 mL) at different concentrations were mixed with 0.6 mL of 4 mM H 2 O 2 solution prepared in phosphate buffer (0.1 M pH 7.4) and incubated for 10 min.

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Evaluation of diuretic and laxative activity of aqueous ...

2021-6-15  to evaluate the extract for its diuretic and laxative potential. Method: The aqueous extract of Argemone mexicana prepared using percolation method and subjected to phytochemical analysis. Evaluation of diuretic and laxative activity was carried out using metabolic cage apparatus and flame photometer as per the standard method reported earlier.

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Phytochemical screening, total phenolic content and in ...

2014-7-8  The total phenolic content of plant extracts was estimated by Folin-Ciocalteau (FC) method as per the procedure of Volluri et al. (2011) with some modifications. Different concentrations of the plant extracts (50-250 μg/mL) and the standard gallic acid (5-25

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Preliminary phytochemical screening, Antibacterial ...

The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azadirachta indica. The antibacterial potential of the methanol leaf extract of the studie

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In vitro Antimicrobial Activity and Phytochemical Analysis ...

The antibacterial activity of 12 plant species extract was assayed in vitro by agar disc diffusion and agar well diffusion method against 6 bacterial species. Table 3 summarizes the microbial growth inhibition of both aqueous and methanol extracts of the screened plant species. The aqueous extract

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In vitro estimation of superfluid critical extracts of ...

2020-7-17  Testing the antimicrobial activity of each plant extract via agar well diffusion method through the formation of clear zones against a wide range of test microorganisms including both Gram-positive and Gram-negative bacteria as well as yeasts. Finally, attempting to primarily identify the constituents of each plant extract using GC–MS.

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In Vivo Diuretic Activity of Hydromethanolic Extract

2020-12-10  The coarsely powdered root bark of C. myricoides was extracted by a cold maceration method using 80% methanol. A portion of the extract was fractionated based on the polarity index of solvents to obtain chloroform, ethyl acetate, and aqueous fractions. To investigate the diuretic activity of the plant, rats were divided into fifteen groups.

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Qualitative analysis of phytochemicals and anti

2020-1-1  The antibacterial potential of the extracts were evaluated on gram-positive (Bacillus subtilis) and gram-negative (Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas pyogens) bacteria in terms of zone of inhibition using agar well diffusion method.In the anti-bacterial screening, it is evident that flower has more anti-bacterial property ...

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Evaluation of antibacteriial and diuretic activity of ...

2018-10-10  500 µg/disc respectively. Amikacin,a standard drug was used as reference control for the screening of antibacterial activity.The extract was found to possess significant diuretic activity with respect to the dose and was found effective against both gram positive and gram negative bacteria in a concentration dependent manner.

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Phytochemical Screening and GC-MS, FT-IR Analysis of ...

2015-9-3  Phytochemical Screening and GC-MS, FT-IR Analysis of Methanolic Extract Leaves of Solanum torvum Sw. ... Solanum torvum Sw. a folk herbal medicinal plant. The plant is sedative and diuretic and the leaves are used as a haemostatic. The ripened fruits are used in ... extract powder was encapsulated in 100 mg of KBr pellet, in order to prepare ...

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PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF

2017-12-21  is diuretic and diaphoretic, it is also used in the treat-ment of syphilis, local inflammation and tumor (17). The plant is used internally in the treatment of indi-gestion, flatulence, constipation and jaundice (16). In this work, the antimicrobial property of the plant leave extracts against some strains of microor-ganisms was evaluated.

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Phytochemical Screening of Root Extract of

Phytochemical screening tests: Phytochemical screening tests were carried out on the crude extract of n-hexane, dichloromethane, dichloromethane/methanol (50:50% by volume) and methanol using standard procedures reported in literature 43-46 to detect the presence of some secondary metabolites namely steroids, terpenoids, saponins, flavonoids, tannins, alkaloids, phenols and glycosides.

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Evaluation of antimicrobial activity of different solvent ...

2020-12-29  Preparation of plant extract . In vivo . leaves of azedarach . M. collected from source plant were washed for 2-3 times with tap water and finally with distilled water, followed by ethanol wash and then allowed to dry at 50. o. Microbiological screening . C for overnight and finally milled to a coarse powder. 100 gm of

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Screening of plant extracts for anthelmintic activity ...

2013-9-5  The methanol extract also showed high anthelmintic activity, with an EC 50 value of 15.9 mg L −1, followed by ethyl acetate and petroleum ether extracts, with EC 50 values of 104.7 and 160.6 mg L −1, respectively. Water extract of C. chinensis exhibited the least activity with the maximum anthelmintic efficacy of 56 % at 300.0 mg L −1.

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Diuretic and serum electrolyte regulation potential of ...

2016-6-3  Therefore, we can suggest that the diuretic potential of the methanolic extract of S. surattense is due to the presence of phenolic caffeic acid and methyl caffeate as secondary metabolites [7, 11]. However, antibacterial effect of S. surattense plant extract against Pseudomonas aeruginosa [ 5 , 16 ] most probably linked with diuretic action of ...

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Possible herb-drug interaction of Morus alba .- a ...

2016-7-20  HPLC method. The plant extract and single bioactive molecule were studied for their inhibition potential on pooled CYP450, as well as recombinant human CYP450 is forms such as CYP3A4, CYP2D6, CYP2C9 and CYP1A2 isozymes. The inhibition potential of the plant extract is higher than the single bioactive molecule. Plant extract and its phyto ...

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Biological Activities and Secondary Metabolite

The boiled extract was used for the phytochemical screening, antioxidant potential, thin layer chromatography, bio-autography, and spot screening. Genomic DNA was extracted to find the DNA protection effect of R. hastatus. RESULTS: Antibacterial results showed that diethyl ether extract has the maximum inhibition of S. pyogenes (9.66 ± 0.57 mm).

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Extraction Methods for Preparation of Bioactive Plant

2017-6-1  Extraction methods for preparation of bioactive plant extracts Authors: Waghmare Deepak Harishchandra 1, Deshmukh Arti Madhukar 2 Savitribai Phule Pune University, Pune 1 Vasantrao Naik Marathwada Agriculture University, Parbhani 2 Extraction is the crucial first step in the analysis of medicinal plants, because it is necessary to extract the desired chemical components from the plant ...

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